PES (polyethersulfone) syringe filters are used in laboratories to filter aqueous and biological fluids. These filters primarily have low protein-binding features, high flow rates, and expansive chemical compatibility. Their effectiveness over time requires proper maintenance and care. Below, we'll examine different methods to maximize the longevity and effectiveness of a PES filter.
Proper storage
The first step in sustaining a PES filter is to store it accurately. Store the filters in a cool, dry place out of direct sunlight. Harsh temperatures and UV light can spoil the material. It will reduce the filter's efficiency and life. Filters should be stored in their original packaging until they are ready to use. This packaging is generally created to protect the filters from environmental elements that could compromise their quality.
Avoid contamination
Contamination is one of the immediate causes of early filter deterioration. Before using the filter, confirm that both the solution to be filtered and the supplies, like syringes, containers, etc., are clean and free from impurities. Particles, matter, or biological impurities can block the filter. It reduces its flow speed and filtration effectiveness. To avoid contaminants during filtration, always use sterile practices when working with sensitive biological samples.
Proper priming and handling
Priming the syringe filter is crucial for achieving optimal performance. Before filtering your sample, flush the filter with a small volume of the solvent or solution you intend to filter. This step removes any air bubbles entrapped in the filter membrane. The filter should be fully soaked for efficient filtration. Do not touch the membrane with your fingers when handling the filter. The oils and impurities from your skin can spoil the filter's efficiency. Always hold the filter by the edges or use tools like tweezers to maintain its goodness.
Using the Proper Syringe Size and Pressure
The size of the syringe for the PES filter should be suitable for the volume of the solution to be filtered. A large syringe can cause excessive pressure on the filter. It can damage the membrane and force contaminants to pass through it. On the other hand, using a too-small syringe may not produce sufficient pressure for effective filtration.
Do not exceed the recommended pressure for the filter. Too much pressure can rupture the filter membrane. The filter will fail and the solution can get easily contaminated. Study the manufacturer’s guidelines for the maximum pressure tolerance and make sure to stay within these limits.
Routine Inspection
Regularly inspect your PES syringe filters before use. Look for any visible damage, like cracks or deformities. A compromised filter can cause ineffective filtration and can contaminate your samples. Discard the filter immediately if you see any damage and replace it with a new one.
Perform a simple integrity test before any critical application. Check for any notable resistance during the filtration process or observe the filtrate for unwanted particles or changes in clarity.
Avoiding reuse
PES syringe filters are normally created for single-use applications. Reusing a clean-looking filter, can lose its integrity and cause cross-contamination between samples. The filter’s performance degrades after the first use. The filtration efficiency is reduced, which can cause possible contamination. Always use a new filter per sample to ensure high-quality results.
Select the Right Filter Pore Size
Select the correct pore size for your application. It is effective for the longevity of the PES filter. The pore size determines the size of particles to be retained by the filter. Using a very small, pore-sized filter can lead to immediate clogging and a reduced flow rate. It can shorten the filter's longevity. Contrarily, a too-large pore-size filter may not effectively remove the desired particles. It will be ineffective filtration.
Depending on the application, the common pore sizes for PES syringe filters range from 0.1 µm to 0.45 µm. A 0.22 µm filter is commonly used for sterilization, while a 0.45 µm filter is frequently used for general filtration tasks. Make sure to choose a filter with an appropriate pore size for your specific application to maximize performance and longevity.
Proper disposal
Finally, it’s critical to discard the used PES syringe filters properly. After use, the filters may contain hazardous or biological materials. They must be handled according to provincial regulations. Disposing of filters in the correct waste collection system ensures compliance with safety regulations. It protects the environment from possible infection and contamination.
Conclusion
Proper care and handling of PES syringe filters is vital for maximizing their performance and life. By taking care of the above-written points, the PES Filter will have a long life and will function efficiently for all your laboratory requirements. Simsii, Inc., is a leading supplier of laboratory filtration systems. They provide high-quality, innovative filters for international and domestic companies, supported by scientists and medical professionals. Get in touch to learn more about economically priced, high-quality syringe filters.
Comments
Post a Comment